2019-02-19 19:28:502019-02-19 19:22:30

Biological materials

Mutant

MGNA-A409 (ypjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/409 NBRP B. subtilis, Japan]

''bshB1'' null mutant and ''[[gene|bshB1]]'' / ''[[gene|bshB2]]'' double null mutant available in [SW|John Helmann] lab

GP87 (''ypjG''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab

BKE22470 ([[gene|bshB1]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG

BKK22470 ([[gene|bshB1]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG

MGNA-A409 (ypjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/409 NBRP B. subtilis, Japan]

''bshB1'' null mutant and ''[[gene|bshB1]]'' / ''[[gene|bshB2]]'' double null mutant available in [SW|John Helmann] lab

GP87 (''ypjG''::''pX2''(''cat'')), available in [SW|Stlke] lab